human tc cell lines tpc 1 cl 0643  (Procell Inc)

Journal: Extracellular Vesicles and Circulating Nucleic Acids

Article Title: A single-sEV analysis identifies plasma EPCAM + sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer

doi: 10.20517/evcna.2025.93

Figure Lengend Snippet: The impact of TC-derived sEVs on TC development in vitro and in vivo . The effects of TC-derived sEVs on TC cell proliferation determined by CCK-8 assays (A and B) or colony formation assays (C and D); Representative images of migration assays (E and F) and invasion assays (G and H) of TC cells subjected to PBS or plasma sEVs from TC patients; (I) Schematic diagram of mouse experiments; (J) TPC-1 tumors were photographed when the tumor-bearing mice were sacrificed; (K) The tumor volume was compared between two groups. The data was analyzed using the Student’s t -test. * P value < 0.05; ** P value < 0.01; *** P value < 0.001; ns: not significant. TC: Thyroid carcinoma; sEVs: small extracellular vesicles; CCK-8: cell counting kit-8; PBS: phosphate-buffered saline; OD450: optical density at 450 nm.

Article Snippet: Human TC cell lines TPC-1 and BCPAP were purchased from Procell Life Science & Technology Co., Ltd. and Fuheng Biology, respectively, and they were authenticated using short tandem repeat (STR) method before experiment.

Techniques: Derivative Assay, In Vitro, In Vivo, CCK-8 Assay, Migration, Clinical Proteomics, Cell Counting, Saline

Journal: Extracellular Vesicles and Circulating Nucleic Acids

Article Title: A single-sEV analysis identifies plasma EPCAM + sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer

doi: 10.20517/evcna.2025.93

Figure Lengend Snippet: The function of EPCAM + sEV. (A) The western blotting analysis of EPCAM in 293T cells stably transfected with shNC or shEPCAM. The sEVs were isolated from 293T cells stably transfected with shNC or shEPCAM, namely shNC-sEV or shEPCAM-sEV, respectively. TPC-1 and BCPAP cells were treated with shNC-sEV or shEPCAM-sEV; (B and C) TC cell proliferation determined by CCK-8 assays; (D and E) TC cell proliferation determined by colony formation assays; Representative images of migration assays (F and G) and invasion assays (H and I) of TC cells subjected to shNC-sEV or shEPCAM-sEV. The data was analyzed using the Student’s t -test. * P value < 0.05; ** P value < 0.01; *** P value < 0.001; ns: not significant. EPCAM: Epithelial cell adhesion molecule; sEVs: small extracellular vesicles; shNC: lentivirus of negative control short hairpin RNA; shEPCAM: lentivirus of EPCAM short hairpin RNA; TC: thyroid carcinoma; CCK-8: cell counting kit-8; OD450: optical density at 450 nm.

Article Snippet: Human TC cell lines TPC-1 and BCPAP were purchased from Procell Life Science & Technology Co., Ltd. and Fuheng Biology, respectively, and they were authenticated using short tandem repeat (STR) method before experiment.

Techniques: Western Blot, Stable Transfection, Transfection, Isolation, CCK-8 Assay, Migration, Negative Control, shRNA, Cell Counting

Journal: Translational Oncology

Article Title: LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

doi: 10.1016/j.tranon.2022.101588

Figure Lengend Snippet: LINC01311 overexpression suppresses TC cell growth in vitro . (A) RT-qPCR analysis of LINC01311 in TC cells (KTC-1, BCPAP, TPC-1, and SW579) and normal cells (Nythy-ori3–1). [** P < 0.001 vs. Nythy-ori3–1; n = 3/group; Dunnett's post hoc test]. (B) RT-qPCR analysis of LINC01311 in TC tissues and normal tissues [** P <0.0001 vs. normal tissues; n = 28/group; Welch's t -test]. (C) LINC01311 localization within the nucleus and the cytoplasm of TC cells was tested by RT-qPCR. (D) RT-qPCR analysis of LINC01311 expression in KTC-1 and TPC-1 cells transfected with Empty vectors or LINC01311-overexpressing vectors (LINC01311-OE). (E) CCK-8 assessment of TC cells transfected with Empty vectors or LINC01311-OE. (F) Western blotting analysis of the expressions of Bcl-2 and Bax in TC cells carrying Empty vectors or LINC01311-OE. (G) Cell apoptosis of TC cells transfected with Empty vectors or LINC01311-OE was examined by Annexin V-FITC/PI-labeled flow cytometry. (H) Scratch wound-healing assay transfected TC cells carrying Empty vectors or LINC01311-OE. [D to H: ** P < 0.001 vs. Empty vector; n = 3/group; Student's t -test].

Article Snippet: Normal human primary thyroid follicular epithelial cell line (Nythy-ori3–1) and one of the TC cell lines (TPC-1) were purchased from Millipore (Sigma, USA).

Techniques: Over Expression, In Vitro, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Western Blot, Labeling, Flow Cytometry, Wound Healing Assay, Plasmid Preparation

Journal: Translational Oncology

Article Title: LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

doi: 10.1016/j.tranon.2022.101588

Figure Lengend Snippet: LINC01311 targets miR-146b-5p . (A) StarBase predicted that miR-146b-5p might bind with LINC01311. (B) Luciferase activity in extracts from KTC-1 and TPC-1 cells co-transfected with LINC01311-WT/MUT luciferase vectors and miR-146b-5p mimic or miR-NC. [** P < 0.001 vs. miR-NC; n = 3/group; Student's t -test]. (C) Ago2 RNA immunoprecipitation (RIP) assay was performed to detect miR-146b-5p and LINC01311 levels in KTC-1 and TPC-1 cells with Ago2. [** P < 0.001 vs. Anti-Ago2; n = 3/group; Student's t -test]. (D) RT-qPCR analysis of miR-146b-5p in TC cells (KTC-1 and TPC-1) and normal cells (Nythy-ori3–1). [** P < 0.001 vs. Nythy-ori3–1; n = 3/group; Dunnett's post hoc test]. (E) RT-qPCR analysis of miR-146b-5p in normal and TC tissues. [** P <0.0001 vs. normal tissues; n = 28/group; Welch's t -test]. (F) Pearson correlation coefficient indicating the correlation of LINC01311 expression with that of miR-146b-5p in TC tissues. (G) RT-qPCR analysis of LINC0311 and miR146b-5p expression in xenograft tumors. [** P < 0.001 vs. Empty vector; n = 5/group; Student's t -test]. (H) LINC01311 overexpressing vectors (LINC01311-OE), Empty vectors, miR-146b-5p mimic (mimic), mimic-NC, and LINC01311-OE (OE)+mimic were transfected into KTC-1 and TPC-1 cells. Relative miR-146b-5p levels in the transfected TC cells were quantified via RT-qPCR analysis. [ ⁎⁎ P < 0.001 vs. Empty vector; ## P < 0.001 vs. mimic-NC; ^^ P < 0.001 vs. LINC01311-OE (OE)+mimic; n = 3/group; Dunnett's post hoc test].

Article Snippet: Normal human primary thyroid follicular epithelial cell line (Nythy-ori3–1) and one of the TC cell lines (TPC-1) were purchased from Millipore (Sigma, USA).

Techniques: Luciferase, Activity Assay, Transfection, Immunoprecipitation, Quantitative RT-PCR, Expressing, Plasmid Preparation

Journal: Translational Oncology

Article Title: LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

doi: 10.1016/j.tranon.2022.101588

Figure Lengend Snippet: LINC01311 interacts with miR-146b-5p to counteract the tumor-suppressive role of LINC01311 in TC cells . KTC-1 and TPC-1 cells were transfected with LINC01311 overexpressing vectors (LINC01311-OE), Empty vectors, miR-146b-5p mimic (mimic), mimic-NC, and LINC01311-OE (OE)+mimic. after 48 h. (A) Cell viability in different groups was tested by CCK-8. (B) Western blot analysis of the different groups using anti-Bax and anti-Bcl-2 antibodies. (C) Analysis of tumor cells migration by scratch wound-healing assay in different groups. [** P < 0.001 when compared to the Empty vectors; ## P < 0.001 when compared to mimic-NC; ^^ P < 0.001 vs. LINC01311-OE (OE)+mimic; n = 3/group; Dunnett's post hoc test].

Article Snippet: Normal human primary thyroid follicular epithelial cell line (Nythy-ori3–1) and one of the TC cell lines (TPC-1) were purchased from Millipore (Sigma, USA).

Techniques: Transfection, CCK-8 Assay, Western Blot, Migration, Wound Healing Assay

Journal: Translational Oncology

Article Title: LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

doi: 10.1016/j.tranon.2022.101588

Figure Lengend Snippet: MiR-146b-5p recognizes IMPA2 and downregulates its expression . (A) The binding sites shared by miR-146b-5p and IMPA2 3′UTR, as predicted by starBase. (B) Dual-luciferase reporter experiment verified the binding of MPA2 3′UTR with miR-146b-5p. [** P < 0.001 vs. miR-NC; n = 3/group; Student's t -test]. (C) RT-qPCR analysis of IMPA2 expression in TC tissues and normal tissues. [** P <0.0001 vs. normal tissues; n = 28/group; Welch's t -test]. (D) RT-qPCR analysis of IMPA2 expression in TC cells (KTC-1 and TPC-1) and normal cells (Nythy-ori3–1). [ ⁎⁎ P < 0.001 vs. Nythy-ori3–1; n = 3/group; Dunnett's post hoc test]. (E) Pearson correlation coefficient revealed the association of IMPA2 expression with that of miR-146b-5p in TC tissues. (F) KTC-1 and TPC-1 cells were transfected with miR-146b-5p mimic (mimic), mimic-NC, Empty vector, IMPA2 overexpressing vectors (IMPA2-OE), and IMPA2-OE (OE)+mimic. Western blotting was performed to estimate IMPA2 expression in the different groups. [ ⁎⁎ P < 0.001 when compared to Empty vector; ## P < 0.001 when compared to mimic-NC; ^^ P < 0.001 when compared to IMPA2-OE (OE)+mimic; n = 3/group; Dunnett's post hoc test].

Article Snippet: Normal human primary thyroid follicular epithelial cell line (Nythy-ori3–1) and one of the TC cell lines (TPC-1) were purchased from Millipore (Sigma, USA).

Techniques: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Transfection, Plasmid Preparation, Western Blot

Journal: Translational Oncology

Article Title: LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

doi: 10.1016/j.tranon.2022.101588

Figure Lengend Snippet: IMPA2 overexpression counteracts miR-146b-5p's oncogenic action in TC cells . KTC-1 and TPC-1 cells were transfected with miR-146b-5p mimic (mimic), mimic-NC, Empty vector, IMPA2 overexpressing vectors (IMPA2-OE), and IMPA2-OE (OE)+mimic. After 48 h. (A) Cell viability in different groups was tested by CCK-8. (B) Western blot analysis of the different groups using anti-Bax and anti-Bcl-2 antibodies. (C) Analysis of tumor cell migration in the different groups by means of the scratch wound-healing assay. [ ⁎⁎ P < 0.001 vs. Empty vector; # P < 0.05, ## P < 0.001 vs. mimic-NC; ^^ P < 0.001 vs. IMPA2-OE (OE)+mimic; n = 3/group; Dunnett's post hoc test].

Article Snippet: Normal human primary thyroid follicular epithelial cell line (Nythy-ori3–1) and one of the TC cell lines (TPC-1) were purchased from Millipore (Sigma, USA).

Techniques: Over Expression, Transfection, Plasmid Preparation, CCK-8 Assay, Western Blot, Migration, Wound Healing Assay